Simonelli Luca, PhD

Publications

• Ultrapotent IgA dimeric antibodies neutralize emerging Omicron variants
  Zuo, F. Cao, Y. Sun, R. Wang, Q. Simonelli, L. Du, L. Bertoglio, F. Schubert, M. Guerra, C. Cavalli, A. Hust, M. Robbiani, D. F. Varani, L. Abolhassani, H. Xie, X. S. Hammarstrom, L. Marcotte, H. Pan-Hammarstrom, Q.
  in J Virol (2024) Vol. ppe0174024
  
• Discovery of a pan anti-SARS-CoV-2 monoclonal antibody with highly efficient infected cell killing capacity for novel immunotherapeutic approaches
  Moussa, D. A. Vazquez, M. Chable-Bessia, C. Roux-Portalez, V. Tamagnini, E. Pedotti, M. Simonelli, L. Ngo, G. Souchard, M. Lyonnais, S. Chentouf, M. Gros, N. Marsile-Medun, S. Dinter, H. Pugniere, M. Martineau, P. Varani, L. Juan, M. Calderon, H. Naranjo-Gomez, M. Pelegrin, M.
  in Emerg Microbes Infect (2024) Vol. pp2432345
  
• Human antibodies in Mexico and Brazil neutralizing tick-borne flaviviruses
  Cervantes Rincon, T. Kapoor, T. Keeffe, J. R. Simonelli, L. Hoffmann, H. H. Agudelo, M. Jurado, A. Peace, A. Lee, Y. E. Gazumyan, A. Guidetti, F. Cantergiani, J. Cena, B. Bianchini, F. Tamagnini, E. Moro, S. G. Svoboda, P. Costa, F. Reis, M. G. Ko, A. I. Fallon, B. A. Avila-Rios, S. Reyes-Teran, G. Rice, C. M. Nussenzweig, M. C. Bjorkman, P. J. Ruzek, D. Varani, L. MacDonald, M. R. Robbiani, D. F.
  in Cell Rep (2024) Vol.43 pp114298
  
• Conversion of monoclonal IgG to dimeric and secretory IgA restores neutralizing ability and prevents infection of Omicron lineages
  Marcotte, H. Cao, Y. Zuo, F. Simonelli, L. Sammartino, J. C. Pedotti, M. Sun, R. Cassaniti, I. Hagbom, M. Piralla, A. Yang, J. Du, L. Percivalle, E. Bertoglio, F. Schubert, M. Abolhassani, H. Sherina, N. Guerra, C. Borte, S. Rezaei, N. Kumagai-Braesch, M. Xue, Y. Su, C. Yan, Q. He, P. Gronwall, C. Klareskog, L. Calzolai, L. Cavalli, A. Wang, Q. Robbiani, D. F. Hust, M. Shi, Z. Feng, L. Svensson, L. Chen, L. Bao, L. Baldanti, F. Xiao, J. Qin, C. Hammarstrom, L. Yang, X. Varani, L. Xie, X. S. Pan-Hammarstrom, Q.
  in Proc Natl Acad Sci U S A (2024) Vol.121 ppe2315354120
  
• Analytical Ultracentrifugation Detects Quaternary Rearrangements and Antibody-Induced Conformational Selection of the SARS-CoV-2 Spike Trimer
  Guerrini, G. Mehn, D. Fumagalli, F. Gioria, S. Pedotti, M. Simonelli, L. Bianchini, F. Robbiani, D. F. Varani, L. Calzolai, L.
  in Int J Mol Sci (2023) Vol.24 pp
  
• Adapting Neutralizing Antibodies to Viral Variants by Structure-Guided Affinity Maturation Using Phage Display Technology
  Peissert, F. Pedotti, M. Corbellari, R. Simonelli, L. De Gasparo, R. Tamagnini, E. Plüss, L. Elsayed, A. Matasci, M. De Luca, R. Cassaniti, I. Sammartino, J. C. Piralla, A. Baldanti, F. Neri, D. Varani, L.
  in Global Challenges (2023) Vol. pp
  
• Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein
  Bianchini, F. Crivelli, V. Abernathy, M. E. Guerra, C. Palus, M. Muri, J. Marcotte, H. Piralla, A. Pedotti, M. De Gasparo, R. Simonelli, L. Matkovic, M. Toscano, C. Biggiogero, M. Calvaruso, V. Svoboda, P. Cervantes Rincon, T. Fava, T. Podesvova, L. Shanbhag, A. A. Celoria, A. Sgrignani, J. Stefanik, M. Honig, V. Pranclova, V. Michalcikova, T. Prochazka, J. Guerrini, G. Mehn, D. Ciabattini, A. Abolhassani, H. Jarrossay, D. Uguccioni, M. Medaglini, D. Pan-Hammarstrom, Q. Calzolai, L. Fernandez, D. Baldanti, F. Franzetti-Pellanda, A. Garzoni, C. Sedlacek, R. Ruzek, D. Varani, L. Cavalli, A. Barnes, C. O. Robbiani, D. F.
  in Sci Immunol (2023) Vol. ppeade0958
  
• A conformational switch controlling the toxicity of the prion protein
  Frontzek, K. Bardelli, M. Senatore, A. Henzi, A. Reimann, R. R. Bedir, S. Marino, M. Hussain, R. Jurt, S. Meisl, G. Pedotti, M. Mazzola, F. Siligardi, G. Zerbe, O. Losa, M. Knowles, T. Lakkaraju, A. Zhu, C. Schwarz, P. Hornemann, S. Holt, M. G. Simonelli, L. Varani, L. Aguzzi, A.
  in Nat Struct Mol Biol (2022) Vol.29 pp831-840
  * Marco Bardelli and Karl Frontzek contributed equally to this work (co-first), Luca Varani is corresponding authors.
• Humoral and cell-mediated response elicited by SARS-CoV-2 mRNA vaccine BNT162b2 e in healthcare workers: a longitudinal observational study
  Cassaniti, I. Bergami, F. Percivalle, E. Gabanti, E. Sammartino, J. C. Ferrari, A. Guy Adzasehoun, K. M. Zavaglio, F. Zelini, P. Comolli, G. Sarasini, A. Piralla, A. Ricciardi, A. Zuccaro, V. Maggi, F. Novazzi, F. Simonelli, L. Varani, L. Lilleri, D. Baldanti, F.
  in Clin Microbiol Infect (2021) Vol. pp
  
• Systematic Development of Peptide Inhibitors Targeting the CXCL12/HMGB1 Interaction
  Sgrignani, J. Cecchinato, V. Fassi, E. M. A. D'Agostino, G. Garofalo, M. Danelon, G. Pedotti, M. Simonelli, L. Varani, L. Grazioso, G. Uguccioni, M. Cavalli, A.
  in J Med Chem (2021) Vol.64 pp13439-13450.
  
• Rational engineering of the lccbeta T. versicolor laccase for the mediator-less oxidation of large polycyclic aromatic hydrocarbons
  Chiado, A. Bosco, F. Bardelli, M. Simonelli, L. Pedotti, M. Marmo, L. Varani, L.
  in Comput Struct Biotechnol J (2021) Vol.19 pp2213-2222
  
• SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface
  Bertoglio, F. Meier, D. Langreder, N. Steinke, S. Rand, U. Simonelli, L. Heine, P. A. Ballmann, R. Schneider, K. T. Roth, K. D. R. Ruschig, M. Riese, P. Eschke, K. Kim, Y. Schackermann, D. Pedotti, M. Kuhn, P. Zock-Emmenthal, S. Wohrle, J. Kilb, N. Herz, T. Becker, M. Grasshoff, M. Wenzel, E. V. Russo, G. Kroger, A. Brunotte, L. Ludwig, S. Fuhner, V. Kramer, S. D. Dubel, S. Varani, L. Roth, G. Cicin-Sain, L. Schubert, M. Hust, M.
  in Nat Com (2021) Vol.12 pp1577
  
• Bispecific IgG neutralizes SARS-CoV-2 variants and prevents escape in mice
  De Gasparo, R. Pedotti, M. Simonelli, L. Nickl, P. Muecksch, F. Lorenzi, J. C. C. Mazzola, F. Magrì, D. Michalcikova, T. Haviernik, J. Honig, V. Cassaniti, I. Percivalle, E. Mrazkova, B. Polakova,N. Fortova, A. Tureckova, J. Iatsiuk, V. Di Girolamo, S. Palus, P. Zudova, D. Bednar, P. Bukova, I. Bianchini, F. Mehn, D. Nencka, R. Strakova, P. Pavlis, O. Rozman, J. Gioria, S. Sammartino, J. C. Giardina, F. Gaiarsa, S. Hammarström, Q. P. Barnes, C. O. Piralla, A. Bjorkman, P. J. Baldanti, F. Calzolai, L. Nussenzweig, M. C. Bieniasz, P. D. Hatziioannou, T. Prochazka, J. Sedlacek, R. Robbiani, D. F. Ruzek, D. Varani, L.
  in Nature (2021) Vol.593 pp424-428
  * D. Robbiani, D. Ruzek, L. Varani are co-correspnding author. Raoul De Gasparo and Mattia Pedotti contributed equally
• A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity
  Bardelli, M. Frontzek, K. Simonelli, L. Pedotti, M. Mazzola, F. Carta, M. Eckhardt, V. D'Antuono, R. Virgilio, T. Gonzalez, S. F. Aguzzi, A. Varani, L.
  in PLoS Pathog (2018) Vol.14 ppe1007335
  
• Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations
  Simonelli, L. Pedotti, M. Bardelli, M. Zerbe, O. Varani, L.
  in Methods Mol Biol (2018) Vol.1785 pp29-51
  
• A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential
  Wang, J. Bardelli, M. Espinosa, D. A. Pedotti, M. Ng, T. S. Bianchi, S. Simonelli, L. Lim, E. X. Y. Foglierini, M. Zatta, F. Jaconi, S. Beltramello, M. Cameroni, E. Fibriansah, G. Shi, J. Barca, T. Pagani, I. Rubio, A. Broccoli, V. Vicenzi, E. Graham, V. Pullan, S. Dowall, S. Hewson, R. Jurt, S. Zerbe, O. Stettler, K. Lanzavecchia, A. Sallusto, F. Cavalli, A. Harris, E. Lok, S. M. Varani, L. Corti, D.
  in Cell (2017) Vol.171 pp229-241 e15
  
• Balance of Anti-CD123 Chimeric Antigen Receptor Binding Affinity and Density for the Targeting of Acute Myeloid Leukemia
  Arcangeli, S. Rotiroti, M. C. Bardelli, M. Magnani, C. F. Biondi, A. Biagi, E. Tettamanti, S. Varani, L.
  in Mol Ther (2017) Vol.25 pp1933-1945
  
• Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery
  Fumagalli, F. Noack, J. Bergmann, T. J. Pisoni, G. B. Fasana, E. Fregno, I. Galli, C. Loi, M. Solda, T. D'Antuono, R. Raimondi, A. Jung, M. Melnyk, A. Schorr, S. Schreiber, A. Simonelli, L. Varani, L. Wilson-Zbinden, C. Zerbe, O. Hofmann, K. Peter, M. Quadroni, M. Zimmermann, R. Molinari, M.
  in Nat Cell Biol (2016) Vol.18 pp1173-1184
  Fumagalli, F. , Noack J. and Bergmann T. contributed equally to this work. * Recommended by the Faculty of 1000
• Specificity, cross-reactivity and function of antibodies elicited by Zika virus infection
  Stettler, K. Beltramello, M. Espinosa, D. A. Cassotta, A. Bianchi, S. Vanzetta, F. Minola, A. Jaconi, S. Mele, F. Foglierini, M. Pedotti, M. Simonelli, L. Dowall, S. Atkinson, B. Percivalle, E. Simmons, C. P. Varani, L. Blum, J. Baldanti, F. Cameroni, E. Hewson, R. Harris, E. Lanzavecchia, A. Sallusto, F.Corti, D.
  in Science (2016) Vol.353 pp823-6
  K. S and M. B contributed equally to this work; D. A. E. ; V. G. ; A. C. ; S. B. and F. V contributed equally to this work; F. S. and D. C contributed equally to this work and are co-corresponding authors.
• Antibody Binding Modulates Conformational Exchange in Domain III of Dengue Virus E Protein
  Moraes, A. H. Simonelli, L. Pedotti, M. Varani, L. Valente, A. P.
  in J Virol (2015) Vol.90 pp1802-11
  * Luca Varani is co-corresponding author
• Unraveling the Efficacy and Safety Profiles of Anti-CD123 Chimeric Antigen Receptors (CARs) in a Model of Acute Myeloid Leukemia Immunotherapy By Investigating CAR Binding Affinity and Density Variables
  Arcangeli, S. Bardelli, M. Tettamanti, S. Simonelli, L. Magnani, C. F. Varani, V. Biondi, A. Biagi, E.
  in 57th ASH Annual Meeting (2015) Vol.126 pp1359
  
• Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus
  Corti, D. Zhao, J. Pedotti, M. Agnihothram, S. Fett, C. Fernandez-Rodriguez, B. Foglierini, M. Agatic, G. Vanzetta, F. Gopal, R. Langrish, C. J. Barrett, N. A. Sallusto, F. Baric, R. S. Varani, L. Zambon, M. Perlman, S. Lanzavecchia, A.
  in Proc Natl Acad Sci U S A (2015) Vol.112 pp10473-8
  D.C. and J.Z. contributed equally to this work.
• Epitope mapping by solution NMR spectroscopy
  Bardelli, M. Livoti, E. Simonelli, L. Moraes, A. Valente, A. P. Varani, L.
  in J Mol Recognit (2015) Vol.28 pp393-400
  * Luca Varani is co-corresponding author
• Rational modification of estrogen receptor by combination of computational and experimental analysis
  Ferrero, V. E. Pedotti, M. Chiado, A. Calzolai, L. Lettieri, T. Varani, L.
  in PLoS One (2014) Vol.9 ppe102658
  
• Rational Engineering of a Human Anti-Dengue Antibody through Experimentally Validated Computational Docking
  Simonelli, L. Pedotti, M. Beltramello, M. Calzolai, L. Sallusto, F. Lanzavecchia, A. Varani, L.
  in PLoS One (2013) Vol.8 ppe55561
  Luca Simonelli, Mattia Pedotti contributed equally to this work.
• Computational docking of antibody-antigen complexes, opportunities and pitfalls illustrated by influenza hemagglutinin
  Pedotti, M. Simonelli, L. Livoti, E. Varani, L.
  in Int J Mol Sci (2011) Vol.12 pp226-51
  * * Luca Simonelli and Mattia Pedotti contributed equally
• The human immune response to Dengue virus is dominated by highly cross-reactive antibodies endowed with neutralizing and enhancing activity
  Beltramello, M. Williams, K. L. Simmons, C. P. Simonelli, L. Quyen, N. T. Sukupolvi-Petty, S. Navarro-Sanchez, E. Young, P. R. de Silva, A. M. Rey, F. A. Varani, L. Whitehead, S. S. Diamond, M. S. Harris, E. Lanzavecchia, A. Sallusto, F.
  in Cell Host Microbe (2010) Vol.8 pp271-83
  Antonio Lanzavecchia and Federica Sallusto are co-corresponding authors.
• Rapid structural characterization of human antibody-antigen complexes through experimentally validated computational docking
  Simonelli, L. Beltramello, M. Yudina, Z. Calzolai, L. Varani, L.
  in J Mol Biol (2010) Vol.396 pp1491-507