Live Cell Analysis

Overview

Three IncuCyte S3 and one IncuCyte SX5 are available for researchers at Bios+ for real-time imaging. This advanced microscope provides fully autonomous, long-term live cell imaging using wide-field phase contrast and green, red, orange, and NIR fluorescence. It includes powerful, user-friendly software for analyzing various in vitro applications such as toxicology, migration, organoids, and wound healing.

Manager

Andrea Rinaldi
andrea.rinaldi@ior.usi.ch
+41 58 666 7041

Key Features

Automated image acquisition and analysis within a standard tissue culture incubator, maintaining precise control of temperature, humidity, and environmental factors like CO₂ and oxygen.
Optics that move to the imaging areas while cell culture vessels remain stationary.
Capability to simultaneously image and analyze up to six assay plates, compatible with standard multi-well plates(including 384-well, 96-well, 48-well, 24-well, 12-well, and 6-well plates), each running different applications in parallel.
Software that generates label-free, time-based growth curves for cells in both 2D and spheroid cultures.
Fluorescence metrics analysis, including Fluorescent Count, Average Area, Total Area, Confluence, Mean Intensity, Average Integrated Intensity, Total Integrated Intensity, and Eccentricity.
High-definition phase contrast optics and four fluorescent wavelengths (red: ex565-605nm, em625-705nm; green: ex440-480nm, em504-544nm).
Automated turret with 4x PLAN, 10x PLAN FLUOR, and 20x PLAN FLUOR objectives.

In vitro image analysis

Available software modules for in vitro image analysis include:

IncuCyte® cell by cell for assessing proliferation
IncuCyte® fluorescent analysis (cell death, coculture)
IncuCyte® Scratch Wound Cell Migration Software Module
IncuCyte® S3 Spheroid Software Module
IncuCyte® S3 organoids Software Module

The Incucyte® Live-Cell Analysis Systems are compatible with a wider range of culture vessels (from 6-well to 384-well plates).

The Live Cell Analysis is managed by the Institute of Oncology Research (IOR), see Live Cell Analysis Facility.

Istituto di Ricerca in Biomedicina

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