Flow Cytometry

Overview

The Flow Cytometry Facility provides investigators with equipment and support for cell sorting (separation), acquisition, and analysis of flow cytometric data with a variety of state-of-art multicolor flow cytometry instruments.

Manager

David Jarrossay
d avid.jarrossay@irb.usi.ch
+41 58 666 7033

Equipment

Cell sorter
- SORP FACSymphony S6 is a conventional flow cytometer and cell sorter equipped with five lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescence detection channels. It can perform high-speed sorting (up to 20, 000 events/sec) with high purity (up to 99%)
- BD FACSDiscover S8 is a spectral flow cytometer and cell sorter that merges full-spectrum fluorescence detection with real-time high-speed imaging for cell analysis and sorting. It features five excitation lasers (349 nm, 405 nm, 488 nm, 561 nm, 637 nm) and up to 78 detector channels, enabling highly multiplexed panels and improved autofluorescence discrimination.
  It has the integrated “CellView” technology that captures morphological and fluorescent images of individual cells at high speed (via PMTs rather than a conventional camera) and allows sorting decisions based on image-based features like protein localization, cell shape or internal structure.

Benchtop analyzers

- FACSymphony A5, equipped with 5 lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescent channels
- LSRFortessa, equipped with four lasers (488, 561, 640, and 405 nm excitation wavelengths) and 16 fluorescence channels
- FACSCantoII (488, 640 and 405 nm lasers-eight colors, equipped with a high throughput system to acquire samples from 96 or 384 well plates
- ImageStream MkII cytometer combines traditional flow cytometry with high-resolution imaging capabilities. It captures detailed images of individual cells as they flow past a camera, allowing simultaneous measurement of fluorescence intensity, morphology, and spatial distribution of cellular components. It’s equipped with 4 laser lines (405, 488, 561 and 642 nm).

Support

Individual training on bench-top analyzers
Cell sorting
Maintenance of all instruments
Assistance with experimental design
Assistance with data analysis
Assistance with troubleshooting

Istituto di Ricerca in Biomedicina

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