Flow Cytometry

Overview

The Flow Cytometry Facility provides investigators with equipment and support for cell sorting (separation), acquisition, and analysis of flow cytometric data with a variety of state-of-art multicolor flow cytometry instruments.

Manager

David Jarrossay
d avid.jarrossay@irb.usi.ch
+41 58 666 7033

Equipment

Cell sorter
- Cell sorting is performed on a SORP FACSymphony S6 equipped with five lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescence detection channels. It can perform high-speed sorting (up to 20, 000 events/sec) with high purity (up to 99%)

Benchtop analyzers
- FACSymphony A5, equipped with 5 lasers (488, 561, 640, 355, and 405 nm excitation wavelengths) and 28 fluorescent channels
- LSRFortessa, equipped with four lasers (488, 561, 640, and 405 nm excitation wavelengths) and 16 fluorescence channels
- FACSCantoII (488, 640 and 405 nm lasers-eight colors, equipped with a high throughput system to acquire samples from 96 or 384 well plates
- FACSCanto I (488 and 640 nm lasers-six colors), equipped with a high throughput system to acquire samples from 96 or 384 well plates

Support

Individual training on bench-top analyzers
Cell sorting
Maintenance of all instruments
Assistance with experimental design
Assistance with data analysis
Assistance with troubleshooting

Istituto di Ricerca in Biomedicina

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