{"id":38498,"date":"2025-05-08T16:23:01","date_gmt":"2025-05-08T14:23:01","guid":{"rendered":"https:\/\/irb.usi.ch\/?p=38498"},"modified":"2025-05-08T16:29:15","modified_gmt":"2025-05-08T14:29:15","slug":"exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna","status":"publish","type":"post","link":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/","title":{"rendered":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA"},"content":{"rendered":"

A paper published in the journal \u201cNature Communications\u201d by the Cejka laboratory shows how EXO1, a nuclease known for its role in DNA end resection in homologous recombination and in DNA mismatch repair, has a nuclease-independent role to promote the nuclease activity of MLH1-MLH3 complex.<\/strong><\/p>\n

Bellinzona – May 08 2025 – During meiosis – a specialized cell division that generates eggs and sperm – chromosomes exchange DNA segments through recombination, which promotes genetic variation. The key DNA cleavage activity is carried by the MLH1-MLH3 (MutL\u03b3) complex, which functions together with the MSH4-MSH5 (MutS\u03b3) complex. These proteins are thought to resolve DNA structures called double Holliday junctions to allow proper chromosome segregation. Our research now demonstrates that EXO1 acts as a scaffold, bridging MLH1-MLH3 with MSH4-MSH5 (MutS\u03b3), and DNA itself. Using biochemical and structural modelling approaches, the team found that EXO1 interacts with both MLH1 and MSH4 and the DNA, stabilizing the machinery for proper DNA incision by MutL\u03b3. The work was primarily carried out by Megha Roy and Aurore Sanchez from the Cejka laboratory at the IRB, in collaboration with Raphael Guerois (Universit\u00e9 Paris-Saclay), combining expertise in AlphaFold.<\/p>\n

Link to the Scientific Article<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

A paper published in the journal \u201cNature Communications\u201d by the Cejka laboratory shows how EXO1, a nuclease known for its role in DNA end resection in homologous recombination and in DNA mismatch repair, has a nuclease-independent role to promote the nuclease activity of MLH1-MLH3 complex. Bellinzona – May 08 2025 – During meiosis – a […]<\/p>\n","protected":false},"author":140,"featured_media":38496,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[16],"tags":[],"class_list":["post-38498","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"yoast_head":"\nEXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI\" \/>\n<meta property=\"og:description\" content=\"A paper published in the journal \u201cNature Communications\u201d by the Cejka laboratory shows how EXO1, a nuclease known for its role in DNA end resection in homologous recombination and in DNA mismatch repair, has a nuclease-independent role to promote the nuclease activity of MLH1-MLH3 complex. Bellinzona – May 08 2025 – During meiosis – a […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/\" \/>\n<meta property=\"og:site_name\" content=\"IRB USI\" \/>\n<meta property=\"article:published_time\" content=\"2025-05-08T14:23:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-05-08T14:29:15+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1280\" \/>\n\t<meta property=\"og:image:height\" content=\"720\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Viviana Granelli\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Scritto da\" \/>\n\t<meta name=\"twitter:data1\" content=\"Viviana Granelli\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minuto\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/\"},\"author\":{\"name\":\"Viviana Granelli\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#\\\/schema\\\/person\\\/e3529a53127a78cf8ead39a83c87f29b\"},\"headline\":\"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA\",\"datePublished\":\"2025-05-08T14:23:01+00:00\",\"dateModified\":\"2025-05-08T14:29:15+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/\"},\"wordCount\":211,\"publisher\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/Slide1-6.jpg\",\"articleSection\":[\"News\"],\"inLanguage\":\"it-IT\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/\",\"name\":\"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/Slide1-6.jpg\",\"datePublished\":\"2025-05-08T14:23:01+00:00\",\"dateModified\":\"2025-05-08T14:29:15+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#primaryimage\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/Slide1-6.jpg\",\"contentUrl\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/Slide1-6.jpg\",\"width\":1280,\"height\":720},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"News\",\"item\":\"https:\\\/\\\/irb.usi.ch\\\/.\\\/news\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#website\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/\",\"name\":\"IRB USI\",\"description\":\"Institute for Research in Biomedicine\",\"publisher\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"it-IT\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#organization\",\"name\":\"IRB USI\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"it-IT\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/IRB-Usi.svg\",\"contentUrl\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/IRB-Usi.svg\",\"width\":98,\"height\":43,\"caption\":\"IRB USI\"},\"image\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#\\\/schema\\\/person\\\/e3529a53127a78cf8ead39a83c87f29b\",\"name\":\"Viviana Granelli\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/","og_locale":"it_IT","og_type":"article","og_title":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI","og_description":"A paper published in the journal \u201cNature Communications\u201d by the Cejka laboratory shows how EXO1, a nuclease known for its role in DNA end resection in homologous recombination and in DNA mismatch repair, has a nuclease-independent role to promote the nuclease activity of MLH1-MLH3 complex. Bellinzona – May 08 2025 – During meiosis – a […]","og_url":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/","og_site_name":"IRB USI","article_published_time":"2025-05-08T14:23:01+00:00","article_modified_time":"2025-05-08T14:29:15+00:00","og_image":[{"width":1280,"height":720,"url":"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg","type":"image\/jpeg"}],"author":"Viviana Granelli","twitter_card":"summary_large_image","twitter_misc":{"Scritto da":"Viviana Granelli","Tempo di lettura stimato":"1 minuto"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#article","isPartOf":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/"},"author":{"name":"Viviana Granelli","@id":"https:\/\/irb.usi.ch\/it\/#\/schema\/person\/e3529a53127a78cf8ead39a83c87f29b"},"headline":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA","datePublished":"2025-05-08T14:23:01+00:00","dateModified":"2025-05-08T14:29:15+00:00","mainEntityOfPage":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/"},"wordCount":211,"publisher":{"@id":"https:\/\/irb.usi.ch\/it\/#organization"},"image":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#primaryimage"},"thumbnailUrl":"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg","articleSection":["News"],"inLanguage":"it-IT"},{"@type":"WebPage","@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/","url":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/","name":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA - IRB USI","isPartOf":{"@id":"https:\/\/irb.usi.ch\/it\/#website"},"primaryImageOfPage":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#primaryimage"},"image":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#primaryimage"},"thumbnailUrl":"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg","datePublished":"2025-05-08T14:23:01+00:00","dateModified":"2025-05-08T14:29:15+00:00","breadcrumb":{"@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#breadcrumb"},"inLanguage":"it-IT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/"]}]},{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#primaryimage","url":"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg","contentUrl":"https:\/\/irb.usi.ch\/images\/Slide1-6.jpg","width":1280,"height":720},{"@type":"BreadcrumbList","@id":"https:\/\/irb.usi.ch\/it\/news\/exo1-promotes-the-meiotic-mlh1-mlh3-endonuclease-through-conserved-interactions-with-mlh1-msh4-and-dna\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/irb.usi.ch\/it\/"},{"@type":"ListItem","position":2,"name":"News","item":"https:\/\/irb.usi.ch\/.\/news\/"},{"@type":"ListItem","position":3,"name":"EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA"}]},{"@type":"WebSite","@id":"https:\/\/irb.usi.ch\/it\/#website","url":"https:\/\/irb.usi.ch\/it\/","name":"IRB USI","description":"Institute for Research in Biomedicine","publisher":{"@id":"https:\/\/irb.usi.ch\/it\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/irb.usi.ch\/it\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"it-IT"},{"@type":"Organization","@id":"https:\/\/irb.usi.ch\/it\/#organization","name":"IRB USI","url":"https:\/\/irb.usi.ch\/it\/","logo":{"@type":"ImageObject","inLanguage":"it-IT","@id":"https:\/\/irb.usi.ch\/it\/#\/schema\/logo\/image\/","url":"https:\/\/irb.usi.ch\/images\/IRB-Usi.svg","contentUrl":"https:\/\/irb.usi.ch\/images\/IRB-Usi.svg","width":98,"height":43,"caption":"IRB USI"},"image":{"@id":"https:\/\/irb.usi.ch\/it\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/irb.usi.ch\/it\/#\/schema\/person\/e3529a53127a78cf8ead39a83c87f29b","name":"Viviana Granelli"}]}},"_links":{"self":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts\/38498","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/users\/140"}],"replies":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/comments?post=38498"}],"version-history":[{"count":2,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts\/38498\/revisions"}],"predecessor-version":[{"id":38505,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts\/38498\/revisions\/38505"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/media\/38496"}],"wp:attachment":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/media?parent=38498"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/categories?post=38498"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/tags?post=38498"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}