{"id":36213,"date":"2024-08-27T14:17:15","date_gmt":"2024-08-27T12:17:15","guid":{"rendered":"https:\/\/irb.usi.ch\/?p=36213"},"modified":"2024-08-27T14:30:30","modified_gmt":"2024-08-27T12:30:30","slug":"sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms","status":"publish","type":"post","link":"https:\/\/irb.usi.ch\/it\/news\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\/","title":{"rendered":"Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms"},"content":{"rendered":"<p><strong>A paper published in the journal \u201cNature Communications\u201d by the Sugimoto, Shinohara and Cejka laboratories shows how Sae2 regulates the nuclease activity of the MRX complex to initiate Homologous Recombination mediated rep\u200bair of DNA breaks. <\/strong><\/p>\n<p>Bellinzona &#8211; July 26 2024 &#8211; DNA double-strand breaks (DSBs) are a threat for genome integrity and cell survival and must be repaired correctly. Key to this process is the Mre11-Rad50-Xrs2 complex (MRX)\u200b, which collaborates\u200b with Sae2 to initiate DSB repair. Using a mix of genetic and biochemical approaches, the authors identify a mutant of the Rad50, K1299R, that helps shed light on how Sae2 regulates the endonuclease and 3\u2019-5\u2019 exonuclease activities of the MRX complex. In particular, the mutant is defective in the Sae2-dependent MRX 3\u2019-5\u2019 exonuclease activity, but not In the endonuclease activity, suggesting that Sae2 controls the two activities in distinct ways. Additionally, the authors uncover how the exonuclease activity of MRX is required for the processing of Spo11-mediated breaks during meiosis but is dispensable during the removal of hairpin structures. This work was a collaboration between Giordano Reginato from the Cejka laboratory at IRB, and the Sugimoto (New Jersey Medical School, Newark, USA) and Shinoara (Kindai University, Osaka, Japan) teams.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.nature.com\/articles\/s41467-024-51493-5\">Link to the Scientific Article<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A paper published in the journal \u201cNature Communications\u201d by the Sugimoto, Shinohara and Cejka laboratories shows how Sae2 regulates the nuclease activity of the MRX complex to initiate Homologous Recombination mediated rep\u200bair of DNA breaks. Bellinzona &#8211; July 26 2024 &#8211; DNA double-strand breaks (DSBs) are a threat for genome integrity and cell survival and [&hellip;]<\/p>\n","protected":false},"author":140,"featured_media":36219,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[16],"tags":[],"class_list":["post-36213","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/news\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms - IRB USI\" \/>\n<meta property=\"og:description\" content=\"A paper published in the journal \u201cNature Communications\u201d by the Sugimoto, Shinohara and Cejka laboratories shows how Sae2 regulates the nuclease activity of the MRX complex to initiate Homologous Recombination mediated rep\u200bair of DNA breaks. Bellinzona &#8211; July 26 2024 &#8211; DNA double-strand breaks (DSBs) are a threat for genome integrity and cell survival and [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/irb.usi.ch\/it\/news\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\/\" \/>\n<meta property=\"og:site_name\" content=\"IRB USI\" \/>\n<meta property=\"article:published_time\" content=\"2024-08-27T12:17:15+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-27T12:30:30+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/irb.usi.ch\/images\/Template_News-6.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1280\" \/>\n\t<meta property=\"og:image:height\" content=\"720\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Viviana Granelli\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Scritto da\" \/>\n\t<meta name=\"twitter:data1\" content=\"Viviana Granelli\" \/>\n\t<meta name=\"twitter:label2\" content=\"Tempo di lettura stimato\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minuto\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/\"},\"author\":{\"name\":\"Viviana Granelli\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#\\\/schema\\\/person\\\/e3529a53127a78cf8ead39a83c87f29b\"},\"headline\":\"Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms\",\"datePublished\":\"2024-08-27T12:17:15+00:00\",\"dateModified\":\"2024-08-27T12:30:30+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/\"},\"wordCount\":219,\"publisher\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/irb.usi.ch\\\/images\\\/Template_News-6.jpg\",\"articleSection\":[\"News\"],\"inLanguage\":\"it-IT\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/\",\"url\":\"https:\\\/\\\/irb.usi.ch\\\/it\\\/news\\\/sae2-controls-mre11-endo-and-exonuclease-activities-by-different-mechanisms\\\/\",\"name\":\"Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms - 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