{"id":23457,"date":"2020-12-09T17:59:21","date_gmt":"2020-12-09T15:59:21","guid":{"rendered":"https:\/\/irb.usi.ch\/?p=23457"},"modified":"2021-01-25T12:43:34","modified_gmt":"2021-01-25T10:43:34","slug":"nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab","status":"publish","type":"post","link":"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/","title":{"rendered":"NLRC5 promotes killing by unconventional Vg9Vd2 cells: a new paper by the Guarda Lab"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"23457\" class=\"elementor elementor-23457\" data-elementor-settings=\"[]\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t<section class=\"has_ae_slider elementor-section elementor-top-section elementor-element elementor-element-109e893 elementor-section-boxed elementor-section-height-default elementor-section-height-default ae-bg-gallery-type-default\" data-id=\"109e893\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"has_ae_slider elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-64ca4c2 ae-bg-gallery-type-default\" data-id=\"64ca4c2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5bb860a elementor-widget elementor-widget-text-editor\" data-id=\"5bb860a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\"><p>For long known to regulate non-immune functions, butyrophilins (BTNs) are emerging as important modulators of immunity. The human BTN3A family \u2013 BTN3A1 in particular \u2013 is essential for the activation of unconventional Vg9Vd2 T cells by phosphoantigens. These phosphorylated metabolites can derive from a dysfunctional mevalonate pathway or microorganisms, such as <em>Mycobacterium tuberculosis.<\/em> In spite of their potential role in antimicrobial and anticancer immunity, little is known about the transcriptional regulation of these genes encoded in the extended major histocompatibility complex (MHC) locus.<\/p><p>In this study, a team of researchers from the Institute for Research in Biomedicine (Universit\u00e0 della Svizzera italiana) together with a team of the University of Freiburg, Germany, and other national and international collaborators, identified an \u201cSXY module\u201d in the promoter of <em>BTN3A1<\/em>&#8211;<em>3<\/em> genes. This regulatory motif is well known to recruit the NOD-like receptors (NLR) CIITA or NLRC5, which are transcriptional regulators of MHC class II or class I genes, respectively. Intriguingly, the SXY motif found in the <em>BTN3A1-3<\/em> gene promoters is atypical in spacing and orientation as compared to the one described in MHC gene promoters. We demonstrate that NLRC5 occupies these promoters and transactivates this unconventional SXY, inducing <em>BTN3A <\/em>gene expression. Along these lines, analyses of cancer cells and of <em>M. tuberculosis<\/em>-infected patients\u2019 blood show a robust correlation between <em>NLRC5<\/em> and <em>BTN3A<\/em> gene transcript abundance. Finally, forcing NLRC5 expression promoted Vg9Vd2 T cell-mediated killing of cancer cells.<\/p><p>This work, published in the Cell press journal \u201c<em>iScience<\/em>\u201d, suggest therefore that NLRC5 manipulation, besides enhancing immunity by conventional cytotoxic T cells, also promotes killing by unconventional Vg9Vd2 T cells. These notions might open new opportunities to modulate Vg9Vd2 T cell-mediated antimicrobial and anticancer immunity.<\/p><p><strong>Article<\/strong><\/p><p><a href=\"https:\/\/www.cell.com\/iscience\/fulltext\/S2589-0042(20)31097-X\">NLRC5 promotes transcription of BTN3A1-3 genes and V\u03b39V\u03b42 T cell-mediated killing<\/a><\/p><p>Dang, A.T., Strietz, J., Zenobi, A., Khameneh, H.J., Brandl, S.M., Lozza, L., Conradt, G., Kaufmann, S.H.E., Reith, W., Kwee, I., Minguet, S., Chelbi, S.T., Guarda, G., ISCIENCE (2021), doi:<a href=\"https:\/\/doi.org\/10.1016\/j.isci.2020.101900\">https:\/\/doi.org\/10.1016\/j.isci.2020.101900<\/a>.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>For long known to regulate non-immune functions, butyrophilins (BTNs) are emerging as important modulators of immunity. The human BTN3A family \u2013 BTN3A1 in particular \u2013 is essential for the activation of unconventional Vg9Vd2 T cells by phosphoantigens. These phosphorylated metabolites can derive from a dysfunctional mevalonate pathway or microorganisms, such as Mycobacterium tuberculosis. In spite [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":23461,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[16],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v15.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>NLRC5 promotes killing by unconventional Vg9Vd2 cells: a new paper by the Guarda Lab - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NLRC5 promotes killing by unconventional Vg9Vd2 cells: a new paper by the Guarda Lab - IRB USI\" \/>\n<meta property=\"og:description\" content=\"For long known to regulate non-immune functions, butyrophilins (BTNs) are emerging as important modulators of immunity. The human BTN3A family \u2013 BTN3A1 in particular \u2013 is essential for the activation of unconventional Vg9Vd2 T cells by phosphoantigens. These phosphorylated metabolites can derive from a dysfunctional mevalonate pathway or microorganisms, such as Mycobacterium tuberculosis. In spite [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\" \/>\n<meta property=\"og:site_name\" content=\"IRB USI\" \/>\n<meta property=\"article:published_time\" content=\"2020-12-09T15:59:21+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-01-25T10:43:34+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/irb.usi.ch\/images\/Greta_iScience_paper_Dec2020-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"601\" \/>\n\t<meta property=\"og:image:height\" content=\"323\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo di lettura stimato\">\n\t<meta name=\"twitter:data1\" content=\"2 minuti\">\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/irb.usi.ch\/it\/#website\",\"url\":\"https:\/\/irb.usi.ch\/it\/\",\"name\":\"IRB USI\",\"description\":\"Institute for Research in Biomedicine\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":\"https:\/\/irb.usi.ch\/it\/?s={search_term_string}\",\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"it-IT\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/#primaryimage\",\"inLanguage\":\"it-IT\",\"url\":\"https:\/\/irb.usi.ch\/images\/Greta_iScience_paper_Dec2020-1.jpg\",\"width\":601,\"height\":323},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/#webpage\",\"url\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\",\"name\":\"NLRC5 promotes killing by unconventional Vg9Vd2 cells: a new paper by the Guarda Lab - IRB USI\",\"isPartOf\":{\"@id\":\"https:\/\/irb.usi.ch\/it\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/#primaryimage\"},\"datePublished\":\"2020-12-09T15:59:21+00:00\",\"dateModified\":\"2021-01-25T10:43:34+00:00\",\"author\":{\"@id\":\"https:\/\/irb.usi.ch\/it\/#\/schema\/person\/1bdd6a1c070b712a183ecb2684c11bc8\"},\"breadcrumb\":{\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/#breadcrumb\"},\"inLanguage\":\"it-IT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"item\":{\"@type\":\"WebPage\",\"@id\":\"https:\/\/irb.usi.ch\/it\/\",\"url\":\"https:\/\/irb.usi.ch\/it\/\",\"name\":\"Home\"}},{\"@type\":\"ListItem\",\"position\":2,\"item\":{\"@type\":\"WebPage\",\"@id\":\"https:\/\/irb.usi.ch\/.\/news\/\",\"url\":\"https:\/\/irb.usi.ch\/.\/news\/\",\"name\":\"News\"}},{\"@type\":\"ListItem\",\"position\":3,\"item\":{\"@type\":\"WebPage\",\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\",\"url\":\"https:\/\/irb.usi.ch\/it\/news\/nlrc5-promotes-killing-by-unconventional-vg9vd2-cells-guarda-lab\/\",\"name\":\"NLRC5 promotes killing by unconventional Vg9Vd2 cells: a new paper by the Guarda Lab\"}}]},{\"@type\":\"Person\",\"@id\":\"https:\/\/irb.usi.ch\/it\/#\/schema\/person\/1bdd6a1c070b712a183ecb2684c11bc8\",\"name\":\"Maryse\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","_links":{"self":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts\/23457"}],"collection":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/comments?post=23457"}],"version-history":[{"count":0,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/posts\/23457\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/media\/23461"}],"wp:attachment":[{"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/media?parent=23457"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/categories?post=23457"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/irb.usi.ch\/it\/wp-json\/wp\/v2\/tags?post=23457"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}