{"id":20829,"date":"2020-08-04T18:30:35","date_gmt":"2020-08-04T16:30:35","guid":{"rendered":"https:\/\/irb.usi.ch\/?p=20829"},"modified":"2020-10-12T16:49:28","modified_gmt":"2020-10-12T14:49:28","slug":"marginal-zone-formation-requires-ackr3-expression-on-b-cells-a-new-paper-by-the-thelen-lab","status":"publish","type":"post","link":"https:\/\/irb.usi.ch\/it\/news\/marginal-zone-formation-requires-ackr3-expression-on-b-cells-a-new-paper-by-the-thelen-lab\/","title":{"rendered":"Marginal zone formation requires ACKR3 expression on B cells : a new paper by the Thelen Lab"},"content":{"rendered":"\t\t
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The marginal zone (MZ) of the spleen contains a specialized set of innate-like B cells that are in direct contact with the blood. This study, published in Cell Reports<\/em>, shows that expression of the atypical chemokine receptor 3 (ACKR3) defines two phenotypically, transcriptionally and functionally distinct, equal-sized populations of mouse MZ B cells (MZBs). The ACKR3-expressing population is required for optimal development and function of the MZ and for the differentiation and positioning of all MZBs. In the absence of ACKR3 expression on B cells the MZ is distorted and the MZBs fail to deliver blood-borne antigens to follicular dendritic cells (FDCs). Effective reconstitution of MZ deficient CD19ko<\/sup> mice with either of the two MZB subsets shows that ACKR3 negative MZBs can differentiate into the more mature ACKR3 positive MZBs, but not vice-versa. The phenotype of CD19ko<\/sup> mice lacking a MZ is readily rescued by the adoptive transfer of ACKR3-sufficient, but not -deficient follicular B cells (FOBs), indicating that ACKR3 expression is essential for the establishment of the MZ developmental niche. Accordingly, the inability of CD19ko<\/sup> mice to respond to T-independent antigen is rescued only when ACKR3-proficient, but not ACKR3-deficient FoBs are transferred. Moreover, ACKR3-deficient FOBs are able to reconstitute the MZ if the niche is pre-established by ACKR3 positive proficient MZBs.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ACKR3 expression marks two distinct marginal zone B (MZBs) cell populations. ACKR3 negative MZBs can differentiate into more mature ACKR3 positive MZBs, but not vice-versa. ACKR3 is required to form a proper marginal zone and to establish the correct microarchitecture of mouse spleen, which is necessary for an effective early immune response to T-independent antigens.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Article<\/strong><\/p>

Marginal Zone Formation Requires ACKR3 Expression on B Cells<\/a><\/p>

Radice E., Ameti R. , Melgrati S. , Thelen S. , Jarrossay D. , Thelen M.<\/p>

In Cell Reports , DOI: 10.1016\/j.celrep.2020.107951<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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This article made the cover of the Cell reports issue. The cover shows the marginal sinus (green) of a mouse spleen section lining the white pulp at B cell follicles (blue). Radice et al. adoptively transferred atypical chemokine receptor 3 expressing (yellow) or not expressing (red) B cells into recipients, which were devoid of the marginal zone. Atypical chemokine receptor 3 expression is required to reconstitute the marginal zone and to mount a humoral immune response. Confocal images were taken and processed by Serena Melgrati and Marcus Thelen.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

The marginal zone (MZ) of the spleen contains a specialized set of innate-like B cells that are in direct contact with the blood. This study, published in Cell Reports, shows that expression of the atypical chemokine receptor 3 (ACKR3) defines two phenotypically, transcriptionally and functionally distinct, equal-sized populations of mouse MZ B cells (MZBs). The […]<\/p>\n","protected":false},"author":4,"featured_media":21156,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[16],"tags":[],"yoast_head":"\nMarginal zone formation requires ACKR3 expression on B cells : a new paper by the Thelen Lab - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/news\/marginal-zone-formation-requires-ackr3-expression-on-b-cells-a-new-paper-by-the-thelen-lab\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Marginal zone formation requires ACKR3 expression on B cells : a new paper by the Thelen Lab - IRB USI\" \/>\n<meta property=\"og:description\" content=\"The marginal zone (MZ) of the spleen contains a specialized set of innate-like B cells that are in direct contact with the blood. 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