{"id":20073,"date":"2020-07-07T10:50:50","date_gmt":"2020-07-07T08:50:50","guid":{"rendered":"https:\/\/irb.usi.ch\/?p=20073"},"modified":"2020-07-07T10:51:19","modified_gmt":"2020-07-07T08:51:19","slug":"new-paper-from-cejkas-lab","status":"publish","type":"post","link":"https:\/\/irb.usi.ch\/it\/news\/new-paper-from-cejkas-lab\/","title":{"rendered":"New paper from Cejka’s Lab"},"content":{"rendered":"

CtIP has emerged as one of the main factors that drives the processing of broken DNA into the homologous recombination pathway. CtIP achieves that by stimulating the activity of two nucleases (MRE11 and DNA2), which resect DNA end creating single-strand DNA overhangs required for the downstream steps in the recombination pathway. Cell lacking CtIP consequently exhibit abrogated DNA end resection and homologous recombination.<\/p>\n

A recent study from the Cejka laboratory at IRB, affiliated with USI, shows that a CtIP variant lacking parts of its central domain is even more efficient than the wild type protein. This unexpected result identified that the central domain of CtIP functions as an inhibitor of DNA end resection, and CtIP can regulate the efficacy of DNA end resection both positively and negatively. The study was published in Nucleic Acids Research.<\/p>\n

\"\"<\/a>
The internal region of CtIP inhibits single strand annealing. Schematic of Sae2 and CtIP protein variants used in this study. Key domains and phosphorylation sites (P) are indicated. Missing amino acid regions in CtIP internal deletion mutants are indicated by a black line.<\/figcaption><\/figure>\n
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ArticleThe internal region of CtIP negatively regulates DNA end resection.<\/a><\/p>\n

Howard, S. M., I. Ceppi, R. Anand, R. Geiger and P. Cejka<\/p>\n

Nucleic Acids Res. 2020<\/p>\n<\/section>\n<\/div>\n<\/article>\n","protected":false},"excerpt":{"rendered":"

CtIP has emerged as one of the main factors that drives the processing of broken DNA into the homologous recombination pathway. CtIP achieves that by stimulating the activity of two nucleases (MRE11 and DNA2), which resect DNA end creating single-strand DNA overhangs required for the downstream steps in the recombination pathway. Cell lacking CtIP consequently […]<\/p>\n","protected":false},"author":1,"featured_media":20075,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[16],"tags":[],"yoast_head":"\nNew paper from Cejka's Lab - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/news\/new-paper-from-cejkas-lab\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"New paper from Cejka's Lab - IRB USI\" \/>\n<meta property=\"og:description\" content=\"CtIP has emerged as one of the main factors that drives the processing of broken DNA into the homologous recombination pathway. CtIP achieves that by stimulating the activity of two nucleases (MRE11 and DNA2), which resect DNA end creating single-strand DNA overhangs required for the downstream steps in the recombination pathway. Cell lacking CtIP consequently […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/irb.usi.ch\/it\/news\/new-paper-from-cejkas-lab\/\" \/>\n<meta property=\"og:site_name\" content=\"IRB USI\" \/>\n<meta property=\"article:published_time\" content=\"2020-07-07T08:50:50+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-07-07T08:51:19+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/irb.usi.ch\/images\/nucleic_acid_res_petr_may2020.png\" \/>\n\t<meta property=\"og:image:width\" content=\"2152\" \/>\n\t<meta property=\"og:image:height\" content=\"1606\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo di lettura stimato\">\n\t<meta name=\"twitter:data1\" content=\"1 minuto\">\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/irb.usi.ch\/it\/#website\",\"url\":\"https:\/\/irb.usi.ch\/it\/\",\"name\":\"IRB USI\",\"description\":\"Institute for Research in Biomedicine\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":\"https:\/\/irb.usi.ch\/it\/?s={search_term_string}\",\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"it-IT\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/irb.usi.ch\/it\/news\/new-paper-from-cejkas-lab\/#primaryimage\",\"inLanguage\":\"it-IT\",\"url\":\"https:\/\/irb.usi.ch\/images\/nucleic_acid_res_petr_may2020.png\",\"width\":2152,\"height\":1606,\"caption\":\"The internal region of CtIP inhibits single strand annealing. 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