{"id":29029,"date":"2022-12-07T11:23:06","date_gmt":"2022-12-07T09:23:06","guid":{"rendered":"https:\/\/irb.usi.ch\/?page_id=29029"},"modified":"2025-11-04T15:57:54","modified_gmt":"2025-11-04T13:57:54","slug":"citometria-a-flusso","status":"publish","type":"page","link":"https:\/\/irb.usi.ch\/it\/servizi-irb\/citometria-a-flusso\/","title":{"rendered":"Citometria a flusso"},"content":{"rendered":"\t\t
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Citometria a Flusso<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Panoramica<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Il laboratorio fornisce ai ricercatori attrezzature e supporto per lo smistamento (separazione) delle cellule, l’acquisizione e l’analisi dei dati citometrici a flusso con una variet\u00e0 di strumenti di citometria a flusso multicolore all’avanguardia.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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David Jarrossay<\/strong>
d<\/a>avid.jarrossay@irb.usi.ch<\/a>
+41 58 666 7033<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Strumenti<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Separazione di cellule in sospensione<\/strong><\/p>

  • SORP FACSymphony S6<\/span><\/b> \u00e8 un citometro a flusso
    convenzionale e separatore di cellule, dotato di cinque laser (lunghezze
    d\u2019onda di eccitazione: 488, 561, 640, 355 e 405 nm) e 28 canali di
    rilevazione della fluorescenza. Pu\u00f2 eseguire separazioni ad alta velocit\u00e0
    (fino a 20.000 eventi\/sec) con elevata purezza (fino al 99%).<\/span><\/li>
  • BD FACSDiscover S8<\/strong> \u00e8 un citometro a flusso spettrale e separatore di cellule che unisce la rilevazione completa dello spettro della fluorescenza con l\u2019acquisizione di immagini ad alta velocit\u00e0 in tempo reale per l\u2019analisi e la separazione cellulare. \u00c8 dotato di cinque laser di eccitazione (349 nm, 405 nm, 488 nm, 561 nm, 637 nm) e fino a 78 canali di rilevazione, permettendo pannelli altamente multiplexati e un miglioramento nella discriminazione dell\u2019autofluorescenza.
    Dispone della tecnologia integrata \u201cCellView\u201d<\/b>, che cattura immagini morfologiche e fluorescenti delle singole cellule ad alta velocit\u00e0 (tramite PMT invece di una fotocamera convenzionale) e consente decisioni di separazione basate su caratteristiche delle immagini come localizzazione delle proteine, forma cellulare o struttura interna.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Citometri a flusso<\/strong><\/p>

      • FACSymphony A5<\/strong>, dotato di 5 laser (488, 561, 640, 355 e 405 nm) e 28 canali fluorescenti<\/li>
      • LSRFortessa<\/strong>, dotato di quattro laser (488, 561, 640 e 405 nm) e 16 canali fluorescenti<\/li>
      • FACSCanto II<\/strong> (laser a 488, 640 e 405 nm \u2013 otto colori), dotato di un sistema ad alto rendimento per acquisire campioni da piastre da 96 o 384 pozzetti<\/li>
      • ImageStream MkII cytometer<\/strong> combina la citometria a flusso tradizionale con capacit\u00e0 di imaging ad alta risoluzione. Cattura immagini dettagliate delle singole cellule mentre passano davanti a una fotocamera, permettendo la misurazione simultanea dell\u2019intensit\u00e0 della fluorescenza, della morfologia e della distribuzione spaziale dei componenti cellulari. \u00c8 dotato di 4 linee laser (405, 488, 561 e 642 nm).<\/li><\/ul><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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        \"SORP
        SORP FACSymphony S6<\/figcaption><\/figure><\/div>
        \"BD
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        SORP FACSymphony A5<\/figcaption><\/figure><\/div>
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        BD FACSCanto II<\/figcaption><\/figure><\/div>
        \"ImageStream
        ImageStream Mkll (3)<\/figcaption><\/figure><\/div>\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t
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        • Formazione individuale sui citometri a flusso<\/li>
        • Separazione di cellule in sospensione<\/li>
        • Manutenzione di tutti gli strumenti<\/li>
        • Assistenza nella progettazione sperimentale<\/li>
        • Assistenza nell’analisi dei dati<\/li>
        • Assistenza in caso di problemi<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          Citometria a Flusso Panoramica Il laboratorio fornisce ai ricercatori attrezzature e supporto per lo smistamento (separazione) delle cellule, l’acquisizione e l’analisi dei dati citometrici a flusso con una variet\u00e0 di strumenti di citometria a flusso multicolore all’avanguardia. Manager David Jarrossaydavid.jarrossay@irb.usi.ch+41 58 666 7033 Strumenti Separazione di cellule in sospensione SORP FACSymphony S6 \u00e8 un citometro […]<\/p>\n","protected":false},"author":137,"featured_media":22621,"parent":22299,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-29029","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nCitometria a flusso - IRB USI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/irb.usi.ch\/it\/servizi-irb\/citometria-a-flusso\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Citometria a flusso - IRB USI\" \/>\n<meta property=\"og:description\" content=\"Citometria a Flusso Panoramica Il laboratorio fornisce ai ricercatori attrezzature e supporto per lo smistamento (separazione) delle cellule, l’acquisizione e l’analisi dei dati citometrici a flusso con una variet\u00e0 di strumenti di citometria a flusso multicolore all’avanguardia. 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